Technical Specifications
Fast MultiSite Mutagenesis System is used for generating mutated PCR fragments by introducing mutation sites on overlapping regions. High fidelity TransStart® FastPfu PCR SuperMix is included for amplification. This kit uses proprietary assembly mix and homologous recombination to seamlessly assemble up to six mutagenesis fragments.
• Fast: Amplified with fast & high-fidelity 2×TransStart® FastPfu PCR SuperMix; only 15 minutes for recombination.
• Flexible: Able to be cloned into any site to realize single-site/ multi-site, continuous/non-continuous mutagenesis.
• Efficient: >90% mutagenesis efficiency.
Notes
We suggest performing 25 cycles for PCR. For low yield PCR products, we suggest using up to 30 cycles.
Storage
at -20°C for two years
Shipping
Dry ice (-70℃)
| Component | FM201-01 (10 rxns) |
| 2×TransStart® FastPfu Fly PCR SuperMix | 1 ml |
| DMT Enzyme (10 units/μl) | 30 μl |
| 2×Assembly Mix | 50 μl |
| DMT Chemically Competent Cell | 10×50 μl |
| Nuclease-free Water | 1 ml |